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. 2020 Nov 27;25(23):5585. doi: 10.3390/molecules25235585

Figure 1.

Figure 1

Monitoring the SELEX progress via ELISA assay. Sub-library pools (biotin-labelled) from the indicated SELEX round were co-incubated with CD63 protein in a 96-well nickel plate at room temperature for 30 min. After 1 h of HRP-labelled anti-biotin antibody incubation, the fluorescence intensity was measured by a plate reader (using the QuantaBlu Fluorogenic Peroxidase Substrate). The binding of each round was calculated after subtracting the mean fluorescent intensity of the binding of the original library to CD63 protein. * p ≤ 0.05.