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. 2020 Nov 27;25(23):5585. doi: 10.3390/molecules25235585

Figure 2.

Figure 2

Evaluation of the binding affinity of aptamers to CD63 protein. (A,A’). The predicted secondary structure of CD63-1 and CD63-2 aptamers using mfold program [36]; (B,B’). Analysing equilibrium dissociation constants (Kd) of CD63-1 aptamer to CD63 molecule by ELISA; (C,C’) Testing binding specificity of aptamers to CD63 with HER2 protein as negative control. All data were derived from triplicate tests. Before binding assay, excessive salmon sperm DNAs (10µg/100µL/test) were employed to block the potential binding site of protein targets. Kd was calculated by GraphPad Prism program 3.03.