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. 2020 Nov 27;25(23):5585. doi: 10.3390/molecules25235585

Figure 3.

Figure 3

Assessment of binding properties of CD63-1 aptamer to CD63-positive cells. (A). FAM-conjugated CD63-1 aptamer was incubated with CD63-positive or -negative cells at 37 °C for 30 min at a concentration of 200 nM, followed by assessing the binding capacity of CD63-1 aptamer to CD63-positive cells with the CD63-negative HEK-293T cells as control; (B). Calculating the equilibrium dissociation constants of CD63-1 aptamer to CD63 positive cells via incubating cells with a series of concentrations of aptamer (0–500 nM). Before binding assay, the potential binding motifs of the protein were blocked via using excessive (50µg/500µL/test) salmon sperm DNAs. Kd was calculated via GraphPad Prism program 3.03.