Table 1.

The recipe of the emulsion PCR.

Oil Phase (µL)
DC5225C	500 µL
DC193	500 µL
CD556	1200 µL
Span 80	80.5 µL
Tween 80	80.5 µL
Tween 20	22.58 µL
Total volume	2383.58 µL
Water phase (PCR master mix, µL)
5× PCR buffer	100 µL
dNTP (10 mM)	10 µL
Primer mix (20uM)	25 µL
Polymerase	10 µL
Template	25 µL
BSA (5%)	5 µL
Water	320 µL
Total volume	500 µL