Figure 5.

Cinnamaldehyde (CA) effect on allergic markers using in vitro models. (A) PCR mRNA quantification of ERK, CXCL-8, COX-2, and p38-MAPK markers in DNP-IgE-sensitized mast cell lines with β-Actin used as an internal control. (B,C) Western blot analysis by an immunoblot of CXCL-8 and COX-2 recorded with a LICOR signal recorder. The blot was segregated with ERK and p38 MAPK with and without the phosphorylated form. (D–F) p-p38 MAPK and p-ERK protein estimation was noted in Dinitrophenyl (DNP)-IgE-sensitized mast cell lines, with data presented as the results of three independent replicate experiments, mean ± S.D. Significant difference is represented as * p < 0.05.