Figure 2.

N-starvation lipophagy strictly depends on the core autophagic machinery (Atg1-Atg9, Atg18, and Vps15), vacuole fusion machinery (Vam7 and Ypt7), and vacuolar proteolysis (proteinases A and B). Cells were normalized in SD-N at OD₆₀₀ 1, and an equal volume of culture (1 mL) was processed at both time-points for all strains to nullify the differential growth (Erg6-GFP dilution) effects in SD-N medium (loading control is not applicable). prA,B: Proteinases A and B-deficient mutant pep4 prb1.