Western blotting for HMGB1, TLR9, and MyD88 in APAP-induced rat hepatotoxicity. The expression level of HMGB1 protein significantly increases at hour 10 (A). TLR9 protein expression does not show a significant increase; however, it tends to increase at hour 10 (B). MyD88 protein expression significantly increases at hour 10 (E). A representative band is shown at each examination point (A, B, and C). GAPDH was used as the loading control (lower panel). Dunnett’s test; *, significantly different from controls at p < 0.05. Bar represents mean ± SD. TLR9, toll-like receptor 9; MyD88, Myeloid Differentiation 88.