Table 2.
Primary antibodies used in immunohistochemistry.
| Antibody (Clone) |
Host | Fixative | Dilution | Pretreatment | Source |
|---|---|---|---|---|---|
| CD68 (ED-1) |
Mouse Monoclonal | PLP solution | 1/500 | 10 µg/mL proteinase K, RT, 10 min | Millipore, Massachusetts, USA |
| CD163 (ED-2) |
Mouse Monoclonal | Acetone/methanol (1:1) | 1/300 | ― | AbD Serotec, Oxford, UK |
| MHC class II (OX-6) | Mouse Monoclonal | PLP solution | 1/300 | Microwave in Citrate buffer, 20 min | AbD Serotec, Oxford, UK |
| CD204 (SRA-E5) |
Mouse Monoclonal | PLP solution | 1/500 | Microwave in Citrate buffer, 20 min | Transgenic Inc, Kumamoto, Japan |
| Iba1 | Rabbit Polyclonal | Formalin | 1/500 | Microwave in Citrate buffer, 20 min | Wako, Osaka, Japan |
| α-SMA (1A4) |
Mouse Monoclonal | Formalin | 1/1,000 | ― | Dako, Glostrup, Denmark |
| HMGB1 | Rabbit Polyclonal | PLP solution | 1/200 | Microwave in Citrate buffer, 20 min | Abcam, Cambridge, UK |
| LC3B | Rabbit Polyclonal | PLP solution | 1/1,000 | Microwave in Citrate buffer, 20 min | SIGMA, St. Louis, USA |
MHC class II, major histocompatibility complex II; α-SMA, alpha-smooth muscle actin; HMGB1, high mobility group box 1; PLP, periodate-lysine-paraformaldehyde.