Figure 1.

RB knocked-down cells repair double strand breaks (DSBs) mainly by the micro-homology mediated end joining (MMEJ) pathway. (A): RB was knocked down by siRNA treatment. (B): Cell cycle distribution in both RB knocked-down cells and the control treated cells. Representative cell cycle profiles are shown from one of the triplicate experiments. (C): Homologous recombination (HR) efficiency was measured by the DRGFP reporter assay. (D): C-NHEJ efficiency was measured by the EJ5GFP reporter assay. (E): MMEJ efficiency was measured by the EJ2GFP reporter assay. All experiments were repeated three times. Bar charts show the mean values from three experiments. Error bars show the standard error of the mean. Unpaired T-test was used for statistical analysis.