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. 2020 Dec 10;10:21738. doi: 10.1038/s41598-020-78895-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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BLI-ISA evaluation of SARS-CoV-2 spike RBD reactivity of pre-pandemic and convalescent plasma. (a,b) Single-dilution BLI-ISA to evaluate the presence of RBD-reactive human antibodies in the pre-pandemic seronegative (SN, cyan) and convalescent seropositive (SP, red) samples compared to no-antigen controls (grey). The assays were performed with plasma at a 1:8 dilution. Bars and dots represent the mean of biological duplicates, and error bars represent one standard deviation from the mean. Blue and green dashed lines represent the mean of seronegative samples plus 3 and 5 standard deviations, respectively. (a) The Total Antibody Binding signal is measured when RBD-biotin-loaded SA biosensors are dipped into plasma samples. (b) The Detection signal is measured when RBD-biotin-loaded SA biosensors that had been dipped into plasma are subsequently dipped into colloidal gold-conjugated anti-human IgG. (c) Dilution series BLI-ISA from representative strong (SP7) and moderate (SP8) seropositive samples. (d) Dilution series BLI-ISA from the weakest seropositive sample (SP3) compared to seronegative plasma samples.