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. 2020 Dec 10;10:21759. doi: 10.1038/s41598-020-78942-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Orthogonal validation by read clustering. Scatter plots between the kallisto|bustools and the starcode workflow show near identical results on the genes targeted by the SwabSeq protocol: (a) RPP30, (b) S, and (c) S spike-in. Each point is a sample and the Pearson correlation is determined for the counts for a gene for all samples between kallisto|bustools and starcode. The code to reproduce this figure is here: https://github.com/pachterlab/BLCSBGLKP_2020/blob/master/notebooks/kb_v_starcode.ipynb.