Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 10;11:6322. doi: 10.1038/s41467-020-20059-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Seven main malignant OS cell subclusters were identified by t-SNE analysis. b Feature plots for marker genes of osteoblastic (COL1A1, RUNX2, and COL3A1) and chondroblastic (ACAN, COL2A1 and SOX9) tumor cells. The color legend shows the log1p normalized expression levels of the genes. c The heatmap of the average expression of top 15 DEGs among six subclusters of osteoblastic tumor cells. The color legend indicates normalized gene expression levels among the subclusters. d The heatmap of GSEA of the 50 hallmark gene sets in MSigDB database among the six osteoblastic cell subclusters. e The scatter plot of the DEGs between osteoblastic tumor cells from lung metastasis (upper panel) or recurrent lesion (lower panel) versus primary lesion. The top 10 DEGs in each comparison were labeled in red. f The t-SNE plot of the four subclusters of chondroblastic cells. g The heatmap of GSEA of the 50 hallmark gene sets in MSigDB among the four chondroblastic clusters. h The scatter plot of the DEGs between chondroblastic and osteoblastic malignant cells. The top 10 genes in each subcluster were marked in red. Relative GSEA scores for each gene set across the cell clusters (d, g) and detailing average normalized gene expression (log1p transformed) values in different tumor sites (e) or types (h) are provided in the Source Data file.