Skip to main content
. 2020 Nov 27;21(23):9015. doi: 10.3390/ijms21239015

Figure 3.

Figure 3

Selection of housekeeping genes (HKGs) for normalization in SH-SY5Y cells. (A) Expression levels of the selected putative HKGs given as quantitative real-time RT-PCR cycle threshold (Cq) values in SH-SY5Y cells after treatment with solvent control, caffeine, theophylline, pentoxifylline, theobromine or propentofylline. The five most stable HKGs are presented in bold letters. (B) Expression stability values for 19 analyzed genes calculated by the NormFinder algorithm. Classification of stability values in most stable genes (0 < stability value < 0.37; green), intermediate genes (0.37 < stability value < 0.53; yellow) and least stable genes (0.53 < stability value < 0.69; red) was done in accordance with Penna et al. [25]. HKGs are ranked from the least to the most stable from left to right and the five most stable genes selected for normalization in SH-SY5Y cells (RN18S1, GPI, TOP1, YWHAZ and GAPDH) are highlighted by a box.