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. 2020 Nov 26;21(23):8983. doi: 10.3390/ijms21238983

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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NFYA protein expression is significantly increased in ML114-treated hPSCs. (A) Real-time PCR data showing no significant increase in NFYA transcript expression caused by 100 μM ML114 (p = 0.39). (B,C) Image of a Coomassie blue-stained SDS-PAGE gel (B) and quantification of whole-lane protein staining (C) show similar gross-protein expression patterns in hPSCs treated with: Control (DMSO-only); 100 μM ML114 fragment (“Fragment”); and 100 μM ML114 (“ML114”). (D,E) Western blot (D) and associated densitometry quantification (E) showing a significant increase in NFYA protein levels in ML114-treated hPSCs compared to the control treatments (n = 3 for all treatments).