Figure 6.

The lapC377fs mutation suppresses the accumulation of LPS precursors and restores the normal LPS synthesis in ΔlapB. Charge-deconvoluted mass spectra in the negative ion mode of LPS from the wild type (A), its ΔlapB (B) and ΔlapB lapC377fs (C) derivatives. Cultures were grown in phosphate-limiting medium at 30 °C. Mass numbers refer to monoisotopic peaks. Mass peaks with rectangular boxes correspond to the glycoform containing the third Kdo. Ovals—derivatives with two Kdo residues with either complete or incomplete core.