Figure 10.

LPS levels are highly reduced in lapC mutants lacking the C-terminal periplasmic domain, which are restored by various suppressor mutations mapping to either lpxC, lapA or lapB genes. Isogenic bacterial cultures of the wild type and its derivatives with the indicated genotype were grown up to an OD₅₉₅ of 0.5 at 30 °C. An equivalent portion of whole cell lysates were applied to a 14% SDS-Tricine gel and transferred by Western blotting. Relative amounts of LPS were revealed by immunoblotting with the LPS-specific monoclonal antibody WN1 222-5 using chemiluminescence detection kit.