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. 2020 Nov 29;21(23):9088. doi: 10.3390/ijms21239088

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Suppressors mapping to the lapB gene that restore growth of lapC mutant bacteria reduce the LapB abundance. (A) Growth of isogenic cultures of strains with lapC190 and with suppressor mutations in the lapB gene was quantified by spot dilution on LA and MacConkey agar at various temperatures. The genotype and incubation temperature are indicated. (B) Immunoblot of total cellular proteins from various strains, used in growth measurement, with LapB-specific antibodies. Purified LapB (lane 10) serves as a control to validate the cross-reactivity of LapB and its position on immunoblot. Equivalent amount of proteins were resolved by SDS-PAGE prior to immunoblotting. (C) The position of various mutations in LapB with the indicated relevant TPR motif are shown in the structure of LapB (PDB 4ZLH, [25]).

Suppressors mapping to the lapB gene that restore growth of lapC mutant bacteria reduce the LapB abundance. (A) Growth of isogenic cultures of strains with lapC190 and with suppressor mutations in the lapB gene was quantified by spot dilution on LA and MacConkey agar at various temperatures. The genotype and incubation temperature are indicated. (B) Immunoblot of total cellular proteins from various strains, used in growth measurement, with LapB-specific antibodies. Purified LapB (lane 10) serves as a control to validate the cross-reactivity of LapB and its position on immunoblot. Equivalent amount of proteins were resolved by SDS-PAGE prior to immunoblotting. (C) The position of various mutations in LapB with the indicated relevant TPR motif are shown in the structure of LapB (PDB 4ZLH, [25]).