Table 3.
The essential lapB gene is dispensable in either lapC190 or lapC377fs backgrounds and the lapC gene becomes non-essential if LpxC is overproduced. ND denotes not done.
| Number of Transductants with Selection for Kanamycin Resistance on Minimal Medium at 30 °C | |||||
|---|---|---|---|---|---|
| Donor | Recipient | ||||
| BW25113 | BW25113 lapC190 | BW25113 lapC377fs |
BW25113 + plpxC+ | ||
| -IPTG | 50 μM IPTG | ||||
| ΔlapA/BKan | 33 KanR small colonies | 2930 KanR normal size | 3470 KanR normal size | ND | ND |
| ΔlapBKan | 36 KanR small colonies | 3154 KanR normal size | 3696 KanR normal size | ND | ND |
| ΔlapCKan | None | ND | ND | None | 754 |