Skip to main content
. 2020 Dec 1;21(23):9163. doi: 10.3390/ijms21239163

Figure 1.

Figure 1

Biological characteristics of KHYG-1 cells used for CD19 single-chain variable fragment (scFv) screening. (A) The effect of cytokines, including IL-2, IL-7, and IL-15, on the proliferation of KHYG-1 cells was investigated. After adding IL-2, IL-7, IL-15, or IL-7/IL-15 to the culture medium, proliferation of KHYG-1 was analyzed by cell counting. (B) Comparison of cell proliferation in KHYG-1 and NK-92. After KHYG-1 and NK-92 were cultured in each culture medium containing IL-2, proliferation was measured by a cell titer-glo assay. (C) Comparison of transduction efficiency in KHYG-1 and NK-92. After luciferase transduction to KHYG-1 and NK-92, luminescence value was detected by bright-glo assay for 6 days. (D) The effect of polybrene on transduction efficiency in KHYG-1. After luciferase transduction with different concentrations of polybrene into KHYG-1, luminescence was measured using a bright-glo assay on the 4th day. (E) KHYG-1 cells were infected with luciferase containing lentiviral particles with different g-values. After 5 days, luminescence was measured. (F) Comparison of gene expression by promoter type in KHYG-1. KHYG-1 cells were transduced with pLVX-CMV-Luc, pLVX-EF1α-Luc, or pLVX-hPGK-Luc lentivirus. On day 4, intensity of luminescence was measured through a bright-glo assay. (G) Antigen-specific cytotoxicity of KHYG-1 and NK-92. Mock KHYG-1, FMC63 CAR KHYG-1, mock NK-92, or FMC63 CAR NK-92 cells (E:T ratio 3:1 or 10:1) were co-cultured with NALM-6 (1.5 × 104 cells), a CD19 positive cell line expressing luciferase, for 5 h. Then, luminescence values were measured.