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. 2020 Dec 1;21(23):9163. doi: 10.3390/ijms21239163

Figure 3.

Figure 3

FMC63 and 4G7 CAR constructs effectively recognize antigen CD19. (A) After transfection of CD19 CAR genes into Jurkat cells, CAR expression was confirmed through the Western blot. (B) CD19 CAR Jurkat (2 × 105 cells) co-cultured with 2 × 104 NALM-6 cells for 21 h. IL-2 level secreted by CD19 CAR Jurkat was measured through ELISA assay. (C) After transduction of eight different CD19 CAR genes into KHYG-1 cells, CAR expression was confirmed through Western blot. (D) Tumor-killing ability of eight different CD19 CAR KHYG-1 cells against luciferase-expressing NALM-6. CD19 CAR KHYG-1 cells (E:T ratio 3:1 or 10:1) were co-cultured with luciferase-expressing NALM-6 (1.5 × 104 cells) for 7 h. Then, luminescence values were measured. (E) FMC63 CAR KHYG-1 or 4G7 CAR KHYG-1 cells were incubated with NALM-6 at different E:T ratios (1:1, 3:1, and 10:1) and different incubation times (6 h and 23 h) to see their lytic activity.