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. 2020 Dec 1;21(23):9163. doi: 10.3390/ijms21239163

Figure 4.

Figure 4

FMC63 and 4G7 CAR T cells effectively responded to CD19-positive NALM-6 cells. (A) After transduction of anti-CD19 CAR lentiviruses into primary human T cells, CAR expression was confirmed through the Western blot. Anti-human CD3 antibody was used to detect CD3ζ of CAR. (B) Tumor-killing ability of FMC63, 4G7, and FITC CAR T cells against luciferase-expressing NALM-6 and U937. FMC63, 4G7, and FITC CAR T cells (2 × 105 cells) were co-cultured with luciferase-expressing NALM-6 (2 × 104 cells) or U937 (2 × 104 cells) for indicated incubation times at the E:T ratio 10:1. Then, luminescence values were measured. (C) FMC63, 4G7, or FITC CAR T cells (1.5 × 105 cells) were co-cultured with NALM-6 cells (1.5 × 104 cells) or U937 cells (1.5 × 104 cells) for 21 h at the E:T ratio 10:1. The amount of IFN-γ and IL-2 secreted by CAR T cells was measured through the ELISA assay.