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. 2020 Dec 3;21(23):9204. doi: 10.3390/ijms21239204

Figure 12.

Figure 12

Immunofluorescence of UCP1/ATP synthase and UCP1/IF1 in BAT following chronic insulin treatment. (A–F) Double labeling with antibodies against UCP1 (green) and ATP synthase (red). (G–L) Double labeling with antibodies against UCP1 (green) and IF1 (red). Harlequin effect is shown with arrowheads. BAT showed various brown adipocytes with different degrees of immunoexpression: UCP1-only brown adipocytes (light-blue star); UCP1 predominantly positive brown adipocytes (green star); brown adipocytes with clear colocalization of UCP1 and ATP synthase/IF1 (yellow star); ATP synthase/IF1 predominantly positive brown adipocytes (pink star); and ATP synthase/IF1-only brown adipocytes (red star). L—lipid body. Nuclei were stained with Sytox Orange. (A–C,G–I), scale bar = 50 µm. (D–F,J–L), scale bar = 10 µm.