Figure 4.
Alterations in membrane properties induced by EcDBS1R4. (A) Effect of EcDBS1R4 on the transmembrane potential of E. coli cells, calculated from the DiOC2(3) green shift. (B) Generalized polarization of LUVs (3 mM lipid) and E. coli cells (1 × 104 cells/mL) labelled with Laurdan, as a function of peptide concentration. (C) Zeta-potential of LUVs (200 µM lipid) and E. coli cells as a function of peptide concentration. (D) Hydrodynamic diameter of LUVs (200 µM lipid), measured by dynamic light scattering as a function of EcDBS1R4 concentration. (E) Fusion/hemifusion efficiency of IML (PE:PG:CL (63:33:4)) LUVs (200 µM lipid) as a function of peptide concentration, calculated using Equation (8). The LUV compositions used were POPC (black), POPC:Chol 70:30 (blue), POPC:POPG 70:30 (red), IML (POPE:POPG:CL 63:33:4) (green) and OML (POPE:POPG:CL:LPS 80:16:1:3) (orange). E. coli cells are represented by grey open circles. All experiments were conducted in triplicate. Data are presented as mean ± SD.