Table 2.
Membrane selectivity parameters obtained and details of the lipid compositions of the systems used.
| Membrane Models Tested | CD | Partition | Quenching | Di-8-ANEPPS | ||||
|---|---|---|---|---|---|---|---|---|
| Composition | Membrane Mimic | Kd,app (μM) | n | K p | IL/IW | Blue Shift (nm) | KSV (M−1) | Kd,app (μM) |
| Free in solution | - | n.d. a | n.d. a | n.a.b | n.a. b | n.a.b | 34.8 ± 0.43 | n.a. b |
| POPC | Control | n.d. a | n.d. a | n.d.a | n.d. a | 0 | 34.2 ± 0.57 | n.d. a |
| POPC:Chol (70:30) | Mammalian cells | n.d. a | n.d. a | 263 ± 158 | 1.4 ± 0.19 | 0 | 28.5 ± 1.1 | n.d. a |
| POPC:POPG (70:30) | General model of bacteria | 75.0 ± 4.0 | 1.8 ± 0.19 | 2064 ± 292 | 2.1 ± 0.05 | 11 | 18.7 ± 4.2 | 15.2 ± 2.6 |
| POPE:POPG:CL (63:33:4) | Inner membrane of E.coli | 44.0 ± 4.0 | 1.4 ± 0.17 | 21615 ± 8407 | 1.0 ± 0.05 | 5 | 5.6 ± 0.14 | 13.4 ± 3.7 |
| POPE:POPG:CL:LPS (80:16:1:3) | Outer membrane of E. coli | n.d. a | n.d. a | n.d a | n.d a | 8.5 | 5.2 ± 0.28 | 9.6 ± 1.4 |
| E. coli ATCC 25922 | - | n.a. b | n.a. b | n.a. b | n.a. b | n.a. b | n.a. b | 1.7 ± 0.49 |
n.d. a—the equation could not fit the data, not possible to determine; n.a. b—data not collected, not applicable.