Figure 4.

Effect of HATMSC2-MVs on the proliferation activity of ovarian cancer cells. (a) Proliferation activity of ES-2 and OAW-42 cells cultured in standard conditions was measured using an MTT assay on day 0, 1, 2, and 3 following treatment with HATMSC2-MVs at different ratios. Untreated cells without MVs served as a control. The data represent mean ± SEM values from four independent experiments performed in triplicate. ** p < 0.01 calculated vs. control on a given day. (b) Representative images from microscopic analysis of the morphology of ovarian cancer cells treated with HATMSC2-MVs at different ratios. ES-2 and OAW-42 cells were co-incubated with HATMSC2-MVs for 72 h. Afterwards, the cells were stained with Calcein AM and images were taken using an inverted microscope (scale bar: 100 µm). HATMSC2-MVs—microvesicles derived from immortalized human mesenchymal stem cells of adipose tissue origin.