Figure 6.

Quantification of cell viability after treatment with HATMSC2-MVs for 72 h at different ratios, determined using flow cytometric analysis of the apoptotic and necrotic cells via the double-staining of ES-2 and OAW-42 cells with propidium iodide and Annexin V. The percentages of alive, early apoptotic, late apoptotic, and necrotic cells were determined using Flowing Software 2. The data represent mean ± SEM values from five independent experiments performed in duplicate. *** p < 0.001, ** p < 0.01 calculated vs. control, ns: non-significant results. HATMSC2-MVs—microvesicles derived from immortalized human mesenchymal stem cells of adipose tissue origin.