Skip to main content
. Author manuscript; available in PMC: 2020 Dec 11.
Published in final edited form as: Int J Hyperthermia. 2020;37(1):66–75. doi: 10.1080/02656736.2019.1707886

Figure 2.

Figure 2.

Efficacy of MNP/AMF hyperthermia alone on the killing of S. aureus biofilm bacteria. (A) The viable CFU numbers of S. aureus in the biofilm following MNP/AMF application as a function of MNPs concentration (1–3 mg/mL) and AMF intensity (18, 22, and 30 kA/m). N = 5–8 per group. N.S: Not Significant (p > .05). #: p < .01. (B) Representative SYTO9/PI fluorescence images following the application of MNP/AMF for varying concentrations of MNPs at 30 kA/m of AMF (green/SYTO9 live cells; red/PI = dead cells). Representative of three independent experiments. (C) Representative flow cytometric plots of forward and side scattering from S. aureus biofilm incubated with varying concentrations of MNPs (0–1 mg/mL) for 2 h. Representative of three independent experiments. (D) The atomic absorption spectroscopy analysis to quantify levels of intracellular iron in the S. aureus bacteria incubated with MNPs (1 mg/mL). N = 3 per group. *p < .05.