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. 2020 Dec 1;21(23):9167. doi: 10.3390/ijms21239167

Figure 3.

Figure 3

Characterization of mAbs secreted by monoclones that were obtained after subcloning of hybridoma #11. (A) ELISA showing reactivity of mAbs from 17 different subclones to RBD purified from E. coli (green), RBD purified from HEK293 cells (blue) and to control protein purified from E. coli (red). (B) Immunoblots of cells transfected with plasmid encoding GFP (G) or the full-length S protein (S). Culture medium from different subclones were used to stain the membranes. Arrow indicates band corresponding to the full-length S-protein.