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. 2020 Dec 1;21(23):9167. doi: 10.3390/ijms21239167

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sequencing of anti-RBD SARS-CoV-2 mAbs. (A) PCR amplification of cDNA encoding immunoglobulins’ k-chain, λ-chain, and heavy-chain from monoclone #11/9. (B) Amino acid sequence of variable domains from light and heavy chains of 11/9 antibodies’. Different regions of immunoglobulins are highlighted: Ig leader sequence (green); framework regions (red); complementarity determining regions (blue); conserved amino acids (bold, underlined). (C) Dendrogram showing clustering of RBD binding antibodies based on the sequences of CDR3 in their light (left) and heavy (right) chains. mAbs #11/9 is indicated with red asterisk.