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. 2020 Nov 27;25(23):5586. doi: 10.3390/molecules25235586

Figure 5.

Figure 5

Effect of AJ-HP-β-CD on the cell cycle and apoptosis in 786-O cells. (A) 786-O cells were treated with PTX (10 nM), AJ (10 nM), and AJ-HP-β-CD (1, 3, 10 nM) for 12 h, and a flow cytometry analysis of the cell cycle assay was performed. (B) 786-O cells were treated with PTX (10 nM), AJ (10 nM), and AJ-HP-β-CD (1, 3, 10 nM) for 24 h. A flow cytometry analysis of apoptosis was performed in each group of cells. (C) The percentage of cells in G1, G2, S phases are shown in the histogram. (D) The percentage of apoptotic cells are quantitatively shown in the histogram, * p < 0.05, ** p < 0.01, *** p < 0.001 compared with vehicle (n = 3), statistical analysis was calculated using student’s t-test (n = 3).