Figure 5.

Expression and phosphorylation of sarcoplasmic reticulum (SR) calcium-handling proteins in atria from JDP2-overexpressing mice. Original Western blots (A,C) and summarized data (B,D) from 8–9 atria isolated from mice overexpressing JDP2 for 5 weeks vs. WT mice (p-values as indicated). Expression of SERCA2a, CSQ and RyR2 was analyzed, as well as phosphorylation of RyR2 at S2808. GAPDH was used as loading control for normalization. Note that the GAPDH signals shown in (A) for SERCA2a and in (C) for pS2808 are identical, as the proteins were derived from the same Western blot.