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. 2020 Dec 3;21(23):9225. doi: 10.3390/ijms21239225

Figure 3.

Figure 3

NFAT5 expression in DMD and unaffected fibroblasts treated for 12 d with hydrocortisone or methylprednisolone NFAT5 mRNA is significantly decreased in DMDFibro after 12 d treatment with 8 mM MP (p < 0.01) and UFibro after 24 h treatment with 8 mM MP (p < 0.001) (A). In Western blotting (WB), three passages are shown per condition (C1 = control passage 1, C2 = control passage 2, C3 = control passage 3, HC1 = hydrocortisone passage 1, HC2 = hydrocortisone passage 2, HC3 = hydrocortisone passage 3, MP1 = methylprednisolone passage 1, MP2 = methylprednisolone passage 2, MP3 = methylprednisolone passage 3). NFAT5 protein is significantly decreased in DMDFibro (p < 0.01 for HC and p < 0.001 for MP) after 24 h of 8 mM HC or 8 mM MP treatment. This decrease remains present after 12 days in both treatment conditions (p < 0.05). GR protein is significantly decreased in DMDFibro (p < 0.05 for HC and p < 0.01 for MP) after 12 days of 8 mM HC or 8 mM MP treatment. The only significant decrease in UFibro is present after 24 h treatment with 8 mM MP (p < 0.001) (B) (n = 3).