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. 2020 Dec 3;21(23):9225. doi: 10.3390/ijms21239225

Figure 6.

Figure 6

NFAT5 and GR colocalization indexes in DMD and unaffected skeletal muscle fibroblasts treated for 24 h with hydrocortisone or methylprednisolone and study of NFAT5 expression after GRsiRNA. Colocalization indexes were established by means of ImageJ. High indexes are observed in DMDFibro treated for 24 h with 8 mM HC and in UFibro treated with 8 mM MP for 24 h (n = 3) (A). After GRsiRNA in DMDFibro and UFibro, NFAT5 is expressed in both cell lines treated for 24 h with 8 mM HC or MP. GR is very weakly present after GRsiRNA compared to control cells (B).