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. 2020 Dec 3;21(23):9209. doi: 10.3390/ijms21239209

Figure 4.

Figure 4

Effects of carvone enantiomers on mucus production in the lung of allergic mice following OVA-induced allergic airway inflammation. (A) Photomicrographs of lungs stained with periodic acid from Schiff (PAS). (B) The mucus production index on PAS-stained slides was determined using the Image J software and expressed as means ± SEM from five animals. + significant difference (p < 0.05) from the unchallenged group; *** (p < 0.001) express significant difference from the untreated OVA-challenged group. Statistical significance was determined with one-way ANOVA and post hoc Tukey test. Twenty-four hours after the last challenge, lungs were fixed in 10% formalin, embedded in paraffin and sectioned. The tissue section slides were stained with PAS. OVA-sensitized mice were treated with R-carvone (10 mg/Kg), S-carvone (10 mg/Kg), dexamethasone (1 mg/Kg) or PBS (vehicle) 1 h prior to each challenge. These analyses were performed by a blind observer using a light microscope with 100× magnifications. Each photomicrograph is representative of two independent experiments performed with five animals per group.