Table 3.

Galactomannan (GM), β-D-glucan and glucuronoxylomannan antigen assays on culture supernatant. For each strain, 5 to 10 colonies incubated at 35 °C for 4 days on Sabouraud media were suspended in 1 ml distilled water. After vigorous agitation, the suspensions were centrifuged for 5 min at 10,000 g. 1, 1:10 and 1:100 dilutions of the supernatants were then tested with Platelia® Aspergillus assay (Bio-Rad, France), Fungitell® assay (Associates of Cape Cod Inc., USA) and Biosynex® CryptoPS assay (Biosynex, France) according to the manufacturer’s recommendations

Isolate	Dilution factor	Galactomannan	β-D-glucan (pg/mL)	Glucuronoxylomannan
HA2	1	> 3,5	> 500	Negative
	10	> 3,5	> 500	n.d.
	100	0,2423	51,048	n.d.
HA3	1	> 3,5	> 500	Negative
	10	> 3,5	> 500	n.d.
	100	0,4197	98,804	n.d.

n.d. Not determined