Fig. 6.

hsa_circ_0001829 promotes GC progression via the miR-155-5p-SMAD2 pathway. a SMAD2 expression in gastric cancer tissues compared with adjacent normal tissues (n = 20, P < 0.05). b Correlations between hsa_circ_0001829 and SMAD2 expression were found with Pearson′ correlation analysis in 20 paired GC tissues (P = 0.001). c-d The mRNA and protein expression level of SMAD2 in GES-1-T and MKN-28 cells with hsa_circ_0001829 knockdown or overexpression by qRT-PCR and western blot. * vs si-NC group, #vs vector group. e-i GES-1-T and MKN-28 cells were transfected with vector, miR-155-5p, hsa_circ_0001829, or miR-155-5p + hsa_circ_0001829. Then the relative mRNA and protein expression of SMAD2 was respectively measured by qRT-PCR analysis (e) and western blot assays (f), and the ability of cell proliferation and invasion was assessed by CCK-8 assay (g), EdU assay (h) and invasion assay (i). *p < 0.05, ** p < 0.01, ^# p < 0.05, ^## p < 0.01