Fig. 4.
Average Intensities utilized to calculate TPEF Autofluorescence Ratios. Average A755/460 intensities of control and pro-calcific mice at 4 and 16 weeks in a leaflets, b commissures and c root. Average A860/525 intensities of control and pro-calcific mice at 4 and 16 weeks in d leaflets, e commissures and f root. Average A810/460 intensities of control and pro-calcific mice at 4 and 16 weeks in g leaflets, h commissures and i root. Average A860/525 intensities of control and pro-calcific mice at 4 and 16 weeks in j leaflets, k commissures and l root. N = 4. *p < 0 .05. TPEF intensity values: Two-way ANOVA with Tukey HSD post hoc test