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. 2020 Dec 3;64(4):3177. doi: 10.4081/ejh.2020.3177

Figure 4.

Figure 4.

YWHAH is a downstream target of miR-519e-5p. A) Putative miR-519e-5p binding sites in the 3’UTR of YWHAH. () A luciferase reporter assay was implemented to certify the association between miR-519e-5p and YWHAH. C-E) RT-qPCR, Western blot and immunofluorescence analyses were used to determine the effects of miR-519e-5p mimic on YWHAH mRNA and protein levels. F) Expression of YWHAH was detected in thyroid cancer cell lines; **p<0.01 vs NC mimic.