Table 1. EC₅₀ (half-maximal inhibitory concentration, HA unit) of AF2240-i and rAF-IL12 in HT29 and 3T3 cells 72-h post-infection.

Column number in 96-well plate	Log10 titre of virus dilution	Observed cytopathic effect (CPE) percentage (%)
		AF2240-i	rAF-IL12
A	0	4/4 × 100 = 100	4/4 × 100 = 100
B	−1	4/4 × 100 = 100	4/4 × 100 = 100
C	−2	4/4 × 100 = 100	4/4 × 100 = 100
D	−3	4/4 × 100 = 100	4/4 × 100 = 100
E	−4	0/4 × 100 = 0	1/4 × 100 = 25
F	−5	0/4 × 100 = 0	0/4 × 100 = 0
G	−6	0/4 × 100 = 0	0/4 × 100 = 0
H	No virus (negative control)	0/4 × 100 = 0	0/4 × 100 = 0

Note:

The TCID₅₀ was calculated based on the Reed and Muench method. The difference of algorithm of AF2240-i-infected cells = (100 − 50)/(100 − 0) = 0.50; log₁₀ 50% end-point dilution = −3 – (0.50) = −3.50; 50% end-point dilution = 10^−3.50; the titre of the virus: 10^3.50 TCID₅₀/0.1 mL = 3.16 × 10⁴ TCID₅₀/mL. The difference of algorithm of rAF-IL12-infected cells = (100 − 50)/(100 − 25) = 0.67; log₁₀ 50% end-point dilution = −3 – (0.67) = −3.67; 50% end-point dilution = 10^−3.67; the titre of the virus: 10^3.67 TCID₅₀/0.1 mL = 4.68 × 10⁴ TCID₅₀/mL. The TCID₅₀ was presented as mean from four replicate determinations.