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. Author manuscript; available in PMC: 2020 Dec 11.
Published in final edited form as: J Med Chem. 2020 Jun 18;63(13):7033–7051. doi: 10.1021/acs.jmedchem.0c00366

Figure 2.

Figure 2.

Diastereomer-selective correction of mitochondrial fragmentation and depolarization in Mfn knockout cells for 13. A and B. Dose-response curves for 13 stereoisomers increasing mitochondrial aspect ratio in cells expressing only Mfn2 (A) or only Mfn1 (B). Prototype mitofusin activator 2 data are shown for comparison. C. Effects of compounds on mitochondrial inner membrane polarization in Mfn1 knockout (KO; top) and Mfn2 KO (bottom) cells. D. Representative confocal imaging of mitochondrial morphology and polarization status in Mfn2 null MEFs treated with different compounds (1 μM, 24 h). Green mitochondria are depolarized and have damaged respiratory function. Scale bars are 10 μm. MT Green (mitotracker green), TMRE (tetramethylrhodamine ethyl ester, red) and nuclear Hoescht (blue) were used for staining. Data are means ± SEM of three independent experiments.