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. 2020 Dec 10;39:279. doi: 10.1186/s13046-020-01797-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Trastuzumab+pertuzumab combination is associated with HER2 downregulation. Cell viability of CTR, T, P, and T + P SkBr3 and BT474 cells treated with 0.1 μg/ml and 1 μg/ml T-DM1, respectively, for 48 h was evaluated by Crystal Violet Assay (a). The results are expressed as percentage of T-DM1-responsive cells relative to control cells, as mean +/− standard deviation. T-DM1 dose-response curves of T and T + P cells are reported for both SkBr3 and BT474 (b). The expression of phosphorylated and total HER2, HER3, EGFR, AKT and ERKs was evaluated by Western Blot (WB) following exposure to 1 μg/ml T-DM1 for 48 h (c). Representative pre- and post-therapy sections from 2 ABC patients, stained by immunohistochemistry with eosin-hematossilin and anti-HER2 antibody (d)