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. 2020 Dec 10;15:72. doi: 10.1186/s13024-020-00419-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Progressive reduction of cortical interneurons in IN-PS cDKO mice. a Left: Nissl staining of comparable sagittal sections of IN-PS cDKO mice and littermate control brains at the ages of 3 and 9 months shows normal gross morphology in the neocortex of IN-PS cDKO mice. Right: Stereological quantification shows similar volume of the neocortex between IN-PS cDKO and control mice at 3 and 9 months of age (F_{1, 24} = 1.74, p = 0.20; 3 M: p > 0.99, 9 M: p = 0.31, two-way ANOVA with Bonferroni’s post hoc comparisons). b Left: Nissl staining of comparable sagittal sections of IN-PS cDKO mice and littermate control brains at the age of 3 and 9 months shows normal gross morphology in the hippocampus of IN-PS cDKO mice. Right: Stereological quantification shows similar volume of the hippocampus between IN-PS cDKO and control mice at 3 and 9 months of age (F_{1, 24} = 0.02, p = 0.88; 3 M: p = 0.31, 9 M: p = 0.32, two-way ANOVA with Bonferroni’s post hoc comparisons). c Left: GAD67 immunostaining of comparable sagittal sections in the neocortex of IN-PS cDKO and littermate control brains at 3 and 9 months of age. Right: Stereological quantification shows age-dependent decreases in the number of GAD67-immunoreactive interneurons in the neocortex of IN-PS cDKO mice compared to that in control mice (F_{1, 24} = 4.89, p = 0.0368; 3 M: Control 2.79 ± 0.12 × 10⁵, cDKO 2.86 ± 0.12 × 10⁵, p > 0.99, 9 M: Control 2.86 ± 0.06 × 10⁵, cDKO 2.40 ± 0.14 × 10⁵, p = 0.0167, two-way ANOVA with Bonferroni’s post hoc comparisons). d Left: GAD67 immunostaining of comparable sagittal sections in hippocampal area CA1 of IN-PS cDKO and littermate control brains at 3 and 9 months of age. Right: Stereological quantification shows no significant decrease in the total number of GAD67-immunoreactive interneurons in the entire hippocampus of IN-PS cDKO mice compared to that in control mice (F_{1, 24} = 0.26, p = 0.61; 3 M: Control 3.84 ± 0.32 × 10⁴, cDKO 3.80 ± 0.08 × 10⁴, p > 0.99; 9 M: Control 3.84 ± 0.22 × 10⁴, cDKO 3.54 ± 0.28 × 10⁴, p = 0.75, two-way ANOVA with Bonferroni’s post hoc comparisons). Scale bar: 100 μm. All data represent mean ± SEM. *p < 0.05. The value in the column indicates the number of mice used in each experiment