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. 2020 Dec 10;15:72. doi: 10.1186/s13024-020-00419-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Progressive decreases of PV+ and SST+ interneurons in the cerebral cortex of IN-PS cDKO mice. a Left: PV immunostaining of comparable sagittal sections in the neocortex of IN-PS cDKO and littermate control brains at 3 and 9 months of age. Right: Stereological quantification shows age-dependent decreases in the number of PV-immunoreactive interneurons in the neocortex of IN-PS cDKO mice compared to that in control mice (F_{1, 24} = 16.20, p = 0.0005; 3 M: Control 1.43 ± 0.02 × 10⁵, cDKO 1.45 ± 0.02 × 10⁵, p > 0.99; 9 M: Control 1.43 ± 0.04 × 10⁵, cDKO 1.09 ± 0.05 × 10⁵, p < 0.0001, two-way ANOVA with Bonferroni’s post hoc comparisons). b Left: SST immunostaining of comparable sagittal sections in the neocortex of IN-PS cDKO and littermate control brains at 3 and 9 months of age. Inserts show higher power views of the boxed areas. Right: Stereological quantification shows age-dependent decreases in the number of SST-immunoreactive interneurons in the neocortex of IN-PS cDKO mice compared to that in control mice (F_{1, 24} = 6.61, p = 0.0168; 3 M: Control 6.17 ± 0.24 × 10⁴, cDKO: 6.28 ± 0.34 × 10⁴, p > 0.99; 9 M: Control 5.84 ± 0.24 × 10⁴, cDKO 4.55 ± 0.24 × 10⁴, p = 0.0027, two-way ANOVA with Bonferroni’s post hoc comparisons). c Left: PV immunostaining of comparable sagittal sections in hippocampus area CA1 (CA1) and the dentate gyrus (DG) of IN-PS cDKO and littermate control brains at 3 and 9 months of age. Right: Stereological quantification shows age-dependent decreases in the number of PV-immunoreactive interneurons in the entire hippocampus of IN-PS cDKO mice compared to that in control mice (F_{1, 24} = 3.84, p = 0.062; 3 M: Control 1.56 ± 0.03 × 10⁴, cDKO 1.58 ± 0.04 × 10⁴, p > 0.99; 9 M: Control 1.59 ± 0.10 × 10⁴, cDKO 1.38 ± 0.03 × 10⁴, p = 0.0244, two-way ANOVA with Bonferroni’s post hoc comparisons). d Left: SST immunostaining of comparable sagittal sections in hippocampus area CA1 (CA1) and the dentate gyrus (DG) of IN-PS cDKO and littermate control brains at 3 and 9 months of age. Inserts show higher power views of the boxed areas. Right: Stereological quantification shows age-dependent decreases in the number of SST-immunoreactive interneurons in the entire hippocampus of IN-PS cDKO mice compared to that in control mice (F_{1, 24} = 20.92, p = 0.0001; 3 M: Control 1.74 ± 0.08 × 10⁴, cDKO 1.85 ± 0.08 × 10⁴, p = 0.44; 9 M: Control 1.63 ± 0.06 × 10⁴, cDKO 1.19 ± 0.04 × 10⁴, p < 0.0001, two-way ANOVA with Bonferroni’s post hoc comparisons). Scale bar: 100 μm. All data represent mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001. The value in the column indicates the number of mice used in each experiment