Fig. 2.

Genetic inhibition of sFRP3 prevents increased microglia activation observed in BubR1^H/H mice. (A) Representative confocal images of ionized calcium-binding adapter molecule 1 (Iba1) (green) and CD68 (red) staining in the hilus of the dentate gyrus in each group. Scale bars: 100 µm (left) and 20 µm (right). (B) Stereological quantification of Iba1⁺ microglia and Iba1⁺ CD68⁺ activated microglia in the molecular layer and the hilus of the dentate gyrus of the hippocampus. Circles within each bar in graphs represent an individual mouse. Values represent mean±standard error of the mean. *P<0.05, **P<0.01, 1-way analysis of variance followed by Tukey post hoc corrections. All groups of mice at 8–10 weeks of age were used at the time of initiating experiments. n.s., not significant; WT, wild-type; HET, heterozygous; KO, knockout.