Figure 10:

Concept of using bacterial membrane-derived DMVs for vaccine development. (A) Schematic demonstration of DMVs for vaccine development. The hypothesis is that the bacterial membrane possesses surface proteins and the LPS of the parent bacteria, and thus the vesicles derived from the bacterial membrane can generate host immunity to bacterial infections. (B) Illustration of DMV preparation. Cultured P aeruginosa bacteria were subject to nitrogen cavitation followed by differential centrifugation to obtain pure DMVs. The structure of DMVs was confirmed by TEM. (C) Experimental design for vaccination using DMVs in the bacterial infection mouse model. (D) The mouse survival after the mice were challenged by a lethal dose of P. aeruginosa (10¹⁰ CFU each, n=10). OMVs (outer membrane vesicles as control). Copyright 2018 Elsevier.