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. 2020 Dec 9;218(3):e20200533. doi: 10.1084/jem.20200533

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© 2020 Kim et al.

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Figure S2. — Unaltered T cell populations and TCR representation in prediabetic NOD.Ocab^fl/flCD4-cre PLNs compared with NOD.Ocab^fl/fl littermate controls. (A) CD3⁺ T cells were isolated and combined from the PLNs of three 8-wk-old NOD.Ocab^fl/flCD4-cre mice or three NOD.Ocab^fl/fl littermate controls. scRNAseq was performed. Clusters were overlaid in UMAP plots. DC, dendritic cell. (B) Six different populations from A were analyzed for differential gene expression. Identified genes are shown as scatter plots. Significantly differentially expressed genes (adjusted P value <0.05) are shown in red. ln, natural logarithm. (C) Percentage contribution of individual variable β chains is shown. (D) Trbv13-3–expressing cells are shown in blue superimposed on the same UMAP scRNAseq data as in A. The effector CD8⁺ T cell cluster is highlighted in red.