Figure S5.

Tat- but not OVA-conjugated peptides concentrate in cultured T cells. CD3⁺ T cells were isolated from the spleens or pancreata of NOD mice and were incubated with 40 µM FITC-conjugated OVA control peptide, or FITC-conjugated to the TAT membrane–penetrating peptide for 15 min. Cells with concentrated FITC fluorescence were observed by epifluorescence microscopy. Images were taken at 40× magnification.