Fig. 3. Data analysis for the A3G–69 nt tail ssDNA complex. (A) The dependence of distance (d) between Domain 1 and Domain 2 on the frame number for the A3G–69 nt tail ssDNA complex. (B) The histogram of distances between the two domains. The mean value for distances (d) between Domain 1 and Domain 2 together with standard deviation is 5.1 ± 1.0 nm. (C) The dependence of the d1 : d2 ratio for the globular structure of A3G on frame number for the A3G–69 nt tail ssDNA complex. (D) The histogram for the d1 : d2 ratio. The mean value for d1 : d2, with the standard deviation, is 1.3 ± 0.2. The data are the result of analysis of ∼600 frames from 10 separate movies (molecules). Each color corresponds to a different molecule.