. Author manuscript; available in PMC: 2020 Dec 11.

Published in final edited form as: Bioorg Med Chem Lett. 2019 Aug 24;29(20):126639. doi: 10.1016/j.bmcl.2019.126639

Table 1.

Anti-influenza A activity in A549 cells and cytotoxicity of compounds 13a-d, 16a-f, 21, 27, 32, 36a-b and 37.

cpd	Anti-Influenza A activity in A549 cells (μM ± SD)		Toxicity (μM) in
	Anti-Influenza A activity in A549 cells (μM ± SD)		PBM	CEM	Vero	Huh7	A549
	EC₅₀	EC₉₀	CC₅₀	CC₅₀	CC₅₀	CC₅₀	CC₅₀
pimodivir	0.004 ± 0.002	0.027 ± 0.01	> 100	48.9	> 100	95.8	20.0
13a	0.007 ± 0.005	0.027 ± 0.01	> 100	91.1	> 100	> 100	≥ 100
13b	0.05 ± 0.04	0.21 ± 0.07	> 100	62.9	> 100	ND	94.6
13c	0.05 ± 0.03	0.28 ± 0.05	> 100	> 100	> 100	> 100	> 100
13d	0.95 ± 0.14	5.1 ± 2.1	23.1	10.1	30.3	14.3	16.2
16a	0.0065 ± 0.0011	0.047 ± 0.008	9.3	4.2	65.9	24.0	14.5
16b	0.017 ± 0.01	0.10 ± 0.06	64.6	31.6	74.9	68.7	14.7
16c	0.029 ± 0.006	0.082 ± 0.01	> 100	> 100	> 100	> 100	> 100
16d	0.027 ± 0.014	0.14 ± 0.11	78.4	12.9	90.3	31.2	41.7
16e	0.4 ± 0.23	2 ± 0.8	>100	29.1	> 100	> 100	≥ 100
16f	>10	ND	>100	> 100	> 100	> 100	> 100
21	>100	ND	84.8	17.3	86.1	70.2	31.3
27	>40	ND	≥ 100	41.7	≥ 100	≥ 100	65.4
32	0.68 ± 0.17	2.5 ± 0.79	> 100	30.8	> 100	71.0	51.9
36a	0.012 ± 0.001	0.036 ± 0.007	> 100	13.7	> 100	62.7	> 100
36b	0.042 ± 0.007	0.14 ± 0.04	> 100	67.0	52.5	70.7	32.5
37	0.016 ± 0.003	0.044 ± 0.011	> 100	48.9	> 100	83.0	30.6

ND: Not determined