Fig 2. Effect of the CHCl₃, EtOAc, water fractions and A. vera gel on HDF migration.

Images were captured at day 0 and showed that an artificial wounded monolayer was created using the scratch assay. Then, treatments were applied with 1 and 3 μg/ml CHCl₃ fraction, 1 and 3 μg/ml EtOAc fraction, 3 and 10 μg/ml water fraction, 1 and 3 μg/ml A. vera gel and control without treatment. Another set of images of fibroblast cell migration were captured at day 1, 2 and 3 after incubation. The cleared areas represented wound and shaded areas resulting in cell migration, which represents the wound closure.